Mayana leaves dissertation

We. Introduction

People of different cultures have extended used crops, especially those with therapeutic principles. They believe plant life to have healing powers. For most parts of the country, a large number of people have appreciated the power of plants in their daily lives. Modern medicine provides downplayed the value of this traditional knowledge, nevertheless it has commenced to acknowledge the value of using plants pertaining to medicinal uses. Plants supply the phytochemicals of the healing program needed by body. Phytochemicals are second metabolites that are scientifically known as the bioactive matters with beneficial activity.

They are nonnutritive herb chemical that have protective, disease-preventing compounds. Detailed screening and analysis of those medicinal plant life may uncover the bioactive components accountable for their healing capacities.

This screening entails processes just like extraction, refinement and portrayal of the active agents of pharmaceutic importance. The Philippines prides itself of it is rich natural resources and its particular flora provides an abundant source of medicinal vegetation. Production and utilization of local plants with phytochemical homes must be given vital importance since there is a great immense possibility that in your area produced and fewer expensive medications can be procured from them.

This may likewise pave the way for the discovery of novel compounds and locally made medicines that can be competitive in the global market. Mayana (coleus sp. ) is known as a traditional flower used to costume cuts and bruises, for that reason, we have chose to do a phytochemical screening. The project desires to15325 find in mayana, worth as a new tool in modern medication at the very least, raise the generalknowledge regarding the plant. The extract will be made by planning a solution of crushed crops in 80 percent ethyl alcoholic beverages.

The tea leaf and stem extract were screened pertaining to the presence of alkaloids, flavonoids and anthraquinones. The assay was done by the paper dvd method of assay. Different concentrations of the extract will be assayed against different kinds of bacteria. The screening demonstrated that the stems contained considerable quantities of alkaloids. The results of the assay demonstrated that custom holds true in the laboratory as the tea leaf extract strongly showed indications of antibacterial activity. The change was the case for the stems as they were found to aid bacterial expansion instead of messing up it. Future researches might include identification with the specific sort of flavonoids, alkaloids and quinones present around the plant and their specific results. They may as well try to utilize bacterial process of the come to make a mayana stem-based nutritious agar.

Targets

To identify the existence of certain phytochemical constituents within Mayana To screen the phytochemical matters and assess antibacterial activity tests of leaf ingredients of Mayana To determine the antiseptic, antifungal, antiprotozoal, cytotoxic, and phytochemical houses of ethanol extracts of leaves of Mayana

2. Materials and Methods

A. Folkloric Study

Before proceeding with our choice of plant, we did a survey to determine the plants that are used by an “albularyo or quack doctor in treating his/her patients. A folkloric review has been prepared by the research workers and the concerns used received by the professor. The folkloric survey well prepared aims to decide the a) the crops that the albularyo still uses at present, b) the medicinal purposes of the people plants stated by the albularyo, c) fault the plant which is used by the albularyo and d) the proper technique of using the flower medicinally plus the recommended serving. We searched for an albularyo in local/rural areas. The community that we decided to go with is located in Bataan.

B. Phytochemical Screening

Plant Collection and Planning

We collected about about 50g of mayana leaves through the local or perhaps rural area. We cleaned it with clean drinking water and drained off the excess liquid. After, we air dried them for just one week or perhaps until the leaves are frail making sure that they are constantly oxygenated to prevent the accumulation of fungi around the leaves. Following air drying, the leaves were cut in to small parts. The small items of leaves were soaked for 2 days in a 500 ml beaker that contain 200 ml ethanol.

Flower Extraction

After 2 days of soaking the leaves, we all extracted the leaves employing filtration. All of us used a glass channel with a filtration paper that is placed on a tripod for easier make use of. A beaker was used to get the extract. The leaves were filtered in the direct with the filtration system paper. Following filtration, all of us obtained the filtrate inside the beaker. The first level of the liquid was taken out through decantation until the draw out was acquired and looking forward to analysis.

Identity of Plant Constituents by Thin Level Chromatography

The extract that people obtained through decantation was tested pertaining to the presence of phytochemicals using thing layer chromatography. A TLC plate was handed to us which procedures 2 cm x several cm. Utilizing a pencil, we marked the TLC dish 1 centimeter from the top rated and you cm from the bottom. And then, we applied the plant samples in the bottom tag by blotting through the use of a capillary tube. The blot should be by least two mm in diameter but not more than a few mm because of it to rise up the TLC plate. After, we ready solvent devices to determine the greatest solvent system for the plant get.

In the preparing of the solvent systems, we used hexane and ethyl acetate. There was 3 ratios used: on the lookout for: 1, several: 3 and 6: four. Three TLC plates together with the blotted flower extract were placed inside the developing sections lined with filter paper and covered by a petri dish. The chambers were saturated initially with 12 ml from the solvent making use of the 3 ratios to allow the solvent to move up the lining when covered prior to placing the blotted TLC discs.

After, thethree TLC china were located inside the three or more developing rooms and it absolutely was kept protected until the solvent climbed up and come to the upper draw. At first, ours didn’t demonstrate any results and did a number of tests. Finally, the blotted TLC menu in the being unfaithful: 1 proportion traveled throughout the TLC. And in our test, the 9: 1 rate was the best solvent system for the sample.

Following obtaining the ideal solvent system to be utilized, we would another developing chamber with the 10 ml solvent. Just like what we performed, we place the 9: 1 ratio (10 ml solvent) in the beaker and layered it with filter newspaper to saturate the chamber. We were offered two TLC plates, the other having three blots and the various other having four blots, a total of eight blots. The TLC dishes were placed in the growing chamber and waited intended for the solvent to reach the upper mark. There is a visible distinguishing or separation of the parts in our TLC plates and then we proceeded with the apply reagent testing.

The TLC plates were cut in seven parts with the eight blots. The professor assigned each group with a apply reagent. Following doing the spray reagent tests, all of us obtained the results in the chromatograms. All of us obtained to get positive results in: Phenols, Coumarins, Anthranoids, and Anthraquinones. The results were drafted in the info sheet presented.

4. Discussion of Results

In the mayana leaves removal, this was the leaves soaked in 2 hundred ml ethanol. From this answer, we attained the plant draw out from the mayana leaves we used for the phytochemical verification. In the second picture, the blotted TLC plates, this was done by making use of the capillary tubes to put the extract inside the TLC menu. At the third picture, this kind of shows the TLC discs in the growing chamber with the 10 milliliters solution which was 9 ml hexane and 1 ml ethanol. Finally, after the spraying of reactants in the TLC plates, we obtained the results above. The positive outcomes that we received are ” Phenols, Coumarins, Anthranoids and Anthraquinones, correspondingly.

According to our results, mayana plant got blue locations which reveal a positive end result with phenols. Phenols include anti-inflammatory and anti-oxidant properties which is the same as the mayana plant. Phenols also have antiseptic and it may be within minimal sum. It also gave a blue zone which tells us it constitutes coumarins. This indicates that it can be used in wounds. It had yellow region which displays positive effect with anthranoids. Anthranoids could be taken once you have constipation which is the same real estate as mayana. Orange-violet color was received in mayana plant which indicates the presence of anthraquinones. Like phenols andanthranoids, you can use it as potent and for congestion. Some scientists have demonstrated that some derivatives have activity in vitro against a few parasitic types.

V. Conclusion

Coleus or perhaps mayana is known as a Southeast Hard anodized cookware plant which includes brightly colored variated leaves that may be popular like a house grow. On the phytochemical screening of this herb, we learned that it provides positive results in coumarins which can be used in wounds, anthranoids which can be a treatment for constipation, phenols which can used as anti-inflammatory and antioxidant and lastly, anthraquinones which can become used since anti-inflammatory and for constipation.

This plant is additionally commonly used as an elaborate plant because of purple leaves. Mayana can easily grow in distinct habitats. It can be one of the usually used folk traditions medicine and it is primarily intended for pain, sore, swelling and cuts and other instances because adjunct medicine for late menstruation. A chemist from the University from the Philippines isolated sterols and triterpenes from leaves of mayana and it showed analgesic, potent and antimicrobial activities. Another interesting component of the plant is its large rosmarinic acid solution content. This compound was noted for its biological activities; prominent of the people are the anti-inflammatory and anti-oxidant homes.

Lastly, we also found away that mayana has its anti-bacterial, anti-fungal, anti-protozoal and cytotoxic components of mayana. Therefore , mayana is a natural medicine that you could use in wounds, headaches, fever and bruises just like precisely what is said in the survey.

VI. Sources

http://scinet.dost.gov.ph/union/ShowSearchResult.php?s=2&f=&p=&x=&page=&sid=1&id=The+phytochemical+screening+and+microbiological+assay+of+Mayana+(%3Cem%3EColeus+blumei%3C%2Fem%3E)+leaves+and+stems&Mtype=PROJECTS http://www.filipinoherbshealingwonders.filipinovegetarianrecipe.com/mayana.htm http://www.stuartxchange.com/Mayana.html

one particular

Tweet