Due to the larger water solubility, certain organic compounds like cresol or oil included phenol mélanges and some mono-aromatic hydrocarbons were considered as basic principle contaminants in groundwater (Flyvbjerg et approach. 1993, Rosenfeld and Straight 1991). p-Cresol or 4-methylphenol [CH3C6H4(OH)] is usually an perfumed compound of phenol derivatives and is generally extracted coming from coal–tar in coal gasification plants by fractionation and various other artificial processes (Muller et al. 2001). p-Cresol is a poisonous chemical, corrosive in character, causes worried system despression symptoms and is also a common by-product produced from tyrosine by a number of anaerobic organisms (Tallur ain al. 2006). Hence, it can be listed being a significant contaminant by the US Environmental Protection Agency (Tallur et ‘s. 2006).
There are reviews on removal/degradation of p-cresol by enzymes, microorganisms and immobilized cellular material of organism(s) (Gunther ain al. 95, Kolomytseva et al. 3 years ago, Muller et al. 2001, Singh ou al. 08, Tallur et al. 06\, 2009, Vermette 2000, Yamada et approach. 2007). Nevertheless , several cons in the immediate microbial detoxing of contaminants were established. The affect of physicochemical and poor bioavailability of contaminants, the existence of other harmful toxins on microbes growth is going to directly affect detoxing (Karigar and Rao 2011). Hence, the enzymatic method has an benefits than bacteria by beating these complications for the detoxification of toxic toxins and is helpful for industrial applications.
You will find reports in copper-containing digestive enzymes like polyphenol oxidases (PPO) which catalyze the oxidation of phenol derivatives inside the presence of O2 (Burton 1994, Rapeanu et al. 2006) and also used for the detoxification of other organic and natural contaminants (Bollag et approach. 2003, Hou et approach. 2011, Rodriguez Couto and Toca Herrera 2006). Yet , PPOs via different sources might change in base specificity and catalytic proficiency (Durán ou al. 2002). Application of immobilization technology may help in increasing the stability of enzyme pertaining to the enhanced detoxing of harmful pollutants and fermentation method.
Numerous matrices are used in enzyme immobilization just like chitosan microspheres, polymeric carrier, polyacrylonitrile beans, magnetic chitosan nanoparticles, nano-porous silica beads and alginate-SiO2 hybrid solution (Dehghanifard ain al. 2013, Jiang ainsi que al. june 2006, Kalkan ain al. 2012, Nicolucci ainsi que al. 2011, Shao et al. 2009, Stanescu ou al. 2012). Although, various previous research used immobilized PPOs for the removal of fabric dyes, non-textile dyes, aqueous phenol and phenol derivatives (Arabaci and Usluoglu 2014, Khan and Husain 2007, Loncar ou al. 2011, Shao et al. 2009), yet, there is not much info available on oxidation of p-cresol by immobilized PPO. With this history, the aim of this study is usually to explore the possibility of p-cresol degradation by Pleurotus sp. isolate VLECK02 PPO immobilized in various matrices like salt alginate (SA), sodium alginate–polyvinyl alcohol (SA–PVA) and SA–PVA–silver nanoparticles (AgNPs).