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Introduction Eugenol is a naturally occurring chemical that has medical applications such as its work with as a organic dental anesthetic. It can be extracted from cloves by using the method of steam handiwork. The extracted compound will then be separated in the water employing methyl chloride, which will in that case be evaporated using a rotary evaporator giving only the essential oil, Eugenol.

VENTOSEAR spectroscopy to be used to determine the structure of the extracted compound. Benefits and Dialogue Four samples of Eugenol had been combined being analyzed to get the final merchandise.

A lower percent recovery, since indicated by Table one particular could be due to the fact that much of the pounds of the cloves was not in fact eugenol. Cloves Used| Eugenol Recovered | Percent Recovery| 10 g| 7. 304 | (7. 304/40) x 100% sama dengan 18. 26%| Table 1: Calculations of the percent recovery of Eugenol. Figure 1: The composition of Eugenol. The IRGI spectroscopy run using the test gave outcomes as demonstrated in Table 2 . The “OH expand accounts for the hydroxyl group on the aromatic ring since shown in Figure 1 )

The C-H peaks happen to be from the mexthoxy group on the aromatic ring. The C=C-H are a result of the hydrogen’s bonded to the carbons of the aromatic ring. The peak in the C=O extend is certainly not from the substance, but may be a result of product contamination. Absorbance | Shape| Intensity| Group-motion| 3512. 21| Broad| Weak| H2O or perhaps “OH stretch| 3072. 52| Sharp| Medium| C=C-H stretch| 2948. 85| Broad| Strong| C-H stretch| 2845. 80| Sharp| Medium| C-H stretch| 1767. 18| Sharp| Weak| C=O stretch| Table 2: IR Table of test taken.

Fresh Extraction of Eugenol started by taking 15 grams of cloves and placing them in a 250mL rounded bottom flask. This flask was in that case attached to a steam handiwork apparatus and heated towards the boiling point of about 100C. The steam was compacted and accumulated in a managed to graduate cylinder. This kind of collected materials consisted of water and the eugenol contained in the cloves. 100mL of the product was collected and placed into a separatory channel. The eugenol now had to be removed from this particular by adding 15mL of CH2Cl2 and shaken.

The levels were permitted to separate. The CH2Cl2 coating was even more dense than water and sank to the bottom in the funnel. The underside layer was collected and another 15mL of dichloromethane was put into the direct to do the process again. This was repeated yet again to secure a final sum of 45mL of dichloromethane with eugenol dissolved in it. The 45mL of product was moved to a 100mL Erlenmyer flask. The next thing was to dry out the mix with zero. 5g of CaSO4 to get rid of any excess drinking water in the blend.

The flask was swirled to allow for the CaSO4 to gather any water. The product was then in order to rest and was subjected to a filter to remove the CaSO4. When filtered, several different trials were accumulated in a single 500mL round lower part flask. This system was then simply placed on to a rotary evaporator to evaporate apart all of the dichloromethane and leave behind only eugenol. This was attained because the hot point of the dichloromethane was much lower than that of eugenol. The final item was after that analyzed by simply IR spectroscopy.

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